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KMID : 1025520070490050593
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Journal of Animal Science and Technology
2007 Volume.49 No. 5 p.593 ~ p.598
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Effects of Sex Steroid Hormones on Differentiation of Pig Preadipocytes
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Kim Hye-Rim
Lee Ki-Ho
Choi In-Ho
Chung Chung-Soo
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Abstract
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The current study was undertaken to determine the effects of sex steroid hormones(estrogen, testosterone and 19-nortestosterone) on differentiation and proliferation of pig preadipocytes. The preadipocytes were isolated from the backfat of new-born female pigs by collagenase digestion. 10－8M and 10－7M sex steroid hormones were treated to the cultured preadipocytes. Sex steroid hormones treated during the early stage of cell growth did not affect differentiation and proliferation of preadipocytes. However, testosterone and 19- nortestosterone treated during the late stage of cell growth stimulated differentiation of pig preadipocytes.
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KEYWORD
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Sex steroid, Pig, Preadipocytes, Differentiation
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